BIOCOMPATIBILITY AND TISSUE REGENERATION LABORATORY

Publications

Books and Monographs
  1. KM Copeland, B Wang, X Shi, D Simionescu, Y Hong, P Bajona, M Sacks. “Decellularization in Heart Valve Tissue Engineering”. Chapter in book: Advances in Heart Valve Biomechanics. M Sacks and J Liao editors, Springer, (2019); pp. 289-317. Abstract

  2. Physiologic and Pathologic Angiogenesis - Signaling Mechanisms and Targeted Therapy. Edited by Dan Simionescu and Agneta Simionescu, InTech (2017). Print ISBN 978-953-51-3023-9. Open Access

  3. L Sierad, A Simionescu, D Simionescu. "Automated Heart Valve Bioreactors for Translational Research". Book chapter in Advances in Heart Valve Biomechanics: The Role of Mechanics in Valvular Physiology, Mechanobiology, and the Bioengineering of Repair and Replacement. Edited by M Sacks and J Liao, Springer, USA, (2016).

  4. Jeremy J. Mercuri, D. Simionescu, “Advances in Tissue Engineering Approaches to Treatment of Intervertebral Disc Degeneration: Cells and Polymeric Scaffolds for Nucleus Pulposus Regeneration”. By invitation. Review in Advances in Polymer Sciences, (Springer Berlin/Heidelberg), special volume on Polymers in Nanomedicine Volume 247, Pages: 201-231. Editors: Kunugi S, Yamaoka T. Kyoto, Japan, (2012). DOI: 10.1007/12_2011_149

  5. G Fercana, D Simionescu. “Polymeric Biomaterials for Vascular Tissue Engineering”. By invitation. In “Polymers for Vascular and Urogenital Applications”. Edited by Shalaby W. Shalaby, K. Burg, and W. Shalaby. Taylor & Francis USA, (2012). ISBN 978-1-4200-7694-3. DOI: 10.1201/b11853-4

  6. Dan Simionescu, Agneta Simionescu, book editors. InTech - Open Access Publisher, Rijeka, Croatia. Title, “Vasculogenesis and Angiogenesis – from Embryonic Development to regenerative Medicine”. ISBN 978-953-307-882-3. (November 2011). Open Access.

  7. Simionescu D. "Artificial heart valves ". By invitation; Encyclopedia of Biomedical Engineering, Metin Akay, editor. John Wiley and Sons, Inc., Hoboken, NJ. 2006. 

  8. Simionescu D, Simionescu A, Deac R, "Molecular pathology of biomaterials, in "Cell adhesion and the extracellular matrix". C. Cruce, A. Simionescu, D. Simionescu et.al., (eds), AIUS Publishers, Craiova, Romania, 1997. (in Romanian).

  9. R. Deac, D. Simionescu, "Heart biomechanics", In BIOMECANICS, 1989, Romanian Academy Press, Bucharest, pp.127-140. (in Romanian).


Selected Refereed Journal Publications (from total of 97 papers)

     Article abstracts may be found online at Pubmed or located using the links below.
     Full text articles can be accessed using the associated links.

  1. Dan Simionescu, Nishanth Tharayil, Elizabeth Leonard, Wenda Carlyle, Alex Schwarz, Kelvin Ning, Christopher Carsten, Juan Carlos Carrillo Garcia, Alexander Carter, Collin Owens, Agneta Simionescu, Binding of Pentagalloyl Glucose to Aortic Wall Proteins: Insights from Peptide Mapping and Simulated Docking Studies, Bioengineering 2023, 10 (8), 936

  2. D Sinha, A Nagy-Mehesz, D Simionescu, JE Mayer Jr, N Vyavahare. Pentagalloyl glucose-stabilized decellularized bovine jugular vein valved conduits as pulmonary conduit replacement. Acta Biomaterialia 2023, 170, 97-110

  3. Marius Mihai Harpa, Sânziana Flămând Oltean, Alexandra Iulia Puscas, Raluca Truta, David Emanuel Anitei, Claudiu Ghiragosian, Cosmin Banceu, Ionela Movileanu, Ovidiu Simion Cotoi, Raluca Niculescu, Horatiu Suciu, Dan Simionescu, Hussam Al Hussein. Bioengineered Small-Diameter Vascular Xenografts as an Alternative to Autologous Vascular Grafting for Emergency Revascularization–a Preliminary Study. Journal of Cardiovascular Emergencies 2023, 9 (4), 103-110

  4. Madjarov JM, Katz MG, Fazal S, Simionescu DT. “External wrapping of ascending aortic dissection with intramural hematoma.” J Thorac Cardiovasc Surg. 2022, 164:(1); e21-22.

  5. Alexandru Filippi, Alina Constantin, Nicoleta Alexandru, Cristina Ana Mocanu, Mihaela Loredana Vlad, Madalina Ioana Fenyo, Agneta Simionescu, Dan Simionescu, Ileana Manduteanu, Adriana Georgescu, (2022) “VLA4 enhanced allogeneic endothelial progenitor cell-based therapy preserves aortic valve function in a mouse model of dyslipidemia and diabetes”. Pharmaceutics, Special Issue on Drug Targeting and Design, Targeted Therapies in Diabetes and Complications, accepted May 12, 2022.

  6. Bo Wang, Leslie N. Sierad, Jeremy J. Mercuri, Agneta Simionescu, Dan T. Simionescu, Lakiesha N. Williams, Ryan Vela, Pietro Bajona, Matthias Peltz, Sharan Ramaswamy, Yi Hong, Jun Liao, “Structural and Biomechanical Characterizations of Acellular Porcine Mitral Valve Scaffolds: Anterior Leaflets, Posterior Leaflets, and Chordae Tendineae, Engineered Regeneration, 2022, ISSN 2666-1381, https://doi.org/10.1016/j.engreg.2022.08.003.

  7. Ileana Manduteanu, Dan Simionescu, Agneta Simionescu, Maya Simionescu. “Molecular Mechanisms and Novel Therapies for Aortic Valve Disease in Diabetes”. Journal of Cellular and Molecular Medicine. Accepted, July 22, (2021).

  8. Ionela Movileanu, Marius Harpa, Hussam Al Hussein, Lucian Harceaga, Alexandru Chertes, Hamida Al Hussein, Georg Lutter, Thomas Puehler, Terezia Preda, Carmen Sircuta, Ovidiu Cotoi, Dan Nistor, Adrian Man, Bogdan Cordos, Radu Deac, Horatiu Suciu, Klara Brinzaniuc, Megan Casco, Leslie Sierad, Margarita Bruce, Dan Simionescu, Agneta Simionescu. “Pre-clinical Testing of Living Tissue Engineered Heart Valves for Pediatric Patients, Challenges and Opportunities”. Journal: Frontiers in Cardiovascular Medicine, section Heart Valve Disease. Article type: Original Research, accepted July (2021). Manuscript ID: 707892

  9. Dan Simionescu, Marius Harpa, Agneta Simionescu, Codruț Oprița, Ionela Movileanu. “Tissue Engineering Heart Valves – a review of more than two decades into preclinical and clinical testing for obtaining the next generation of heart valve substitutes”. Rev Rom Cardiol., 2021. 31(3): 501-510.

  10. Ionela Movileanu, Dan Nistor, Leslie Sierad, Klara Branzaniuc, Codruț Oprița, Marius Harpa, Terezia Preda, Ovidiu Cotoi, Hussam AL Hussein, Hamida AL Hussein, Alexandru Chertes, Horatiu Suciu, Agneta Simionescu, Dan Simionescu. “Developing the tissue engineered heart valve – a descriptive hemodynamic and ultrasound in vitro characterization study of heart valves in a bioreactor”. Rev Rom Cardiol., 2021. 31(3): 555-563.

  11. Cecoltan S, Ciortan L, Macarie RD, Vadana M, Mihaila AC, Tucureanu M, Vlad M-L, Droc I, Gherghiceanu M, Simionescu A, Simionescu DT, Butoi E and Manduteanu I (2021) High Glucose Induced Changes in Human VEC Phenotype in a 3D Hydrogel Derived From Cell-Free Native Aortic Root. Front. Cardiovasc. Med. 8:714573. doi: 10.3389/fcvm.2021.714573

  12. G Lutter, H Dai, J Hansen, D Frank, A Haneya, D Simionescu, J Cremer, T Puehler. “Transcatheter mitral valve replacement (TMVR): annular or apical fixation?”. EuroIntervention (2020), 16:510-517.

  13. Movileanu I, Brinzaniuc K, Harpa M, Nistor D, Cotoi O, Preda T, Hussein H, Moldovan O, Man A, Harceaga L, Sierad L, Simionescu D. “Pressurized Perfusion System for Obtaining Completely Acellular Pulmonary Valve Scaffolds for Tissue Engineering”. ARS Medica Tomitana (2020); 25:4;149-156.

  14. D Simionescu, M Casco, J Turner, N Rierson, J Yue, K Ning. “Chemical Stabilization of The Extracellular Matrix Attenuates Growth of Experimentally Induced Abdominal Aorta Aneurysms in a Large Animal Model”. Journal of Vascular Surgery (2020); 1; 69-80.

  15. A Filippi, A Constantin, N Alexandru, G Voicu, C Constantinescu, D Rebleanu, M Fenyo, D Simionescu, A Simionescu, I Manduteanu, A Georgescu. “Integrins α4β1 and αVβ3 are reduced in endothelial progenitor cells from diabetic dyslipidemic mice and may represent new targets for therapy in aortic valve disease”. Cell Transplantation (2020); 29;1-8.

  16. H Al Hussein, H. Al Hussein, D Simionescu, C Sircuta, O S Cotoi, I Movileanu, D Nistor, B Cordos, R Deac, H Suciu, K Brinzaniuc, M M Harpa. “Challenges in perioperative animal care for orthotopic implantation of tissue-engineered pulmonary valves in the ovine model.” Tissue Engineering and Regenerative Medicine. (2020); 17; 847-862.

  17. Al Hussein Hussam, Al Hussein Hamida*, Harpa M, Sircuta C, Cotoi O, Man A, Deac R, Suciu H, Brinzaniuc K, Simionescu D. “Subcoronary Technique for Orthotopic Implantation of Aortic Scaffold Reinforced with Polytetrafluoroethylene Strips in Sheep: A pilot Study”. Mater. Plast. 2020; 57: 104-112.

  18. JM Madjarov, MG Katz, S Fazal, D Simionescu. “External wrapping of ascending aortic dissection with intramural hematoma. J Thoracic and Cardiovascular Surgery (2020). e1-e2.

  19. Filippi A, Constantin A, Alina Constantin, Nicoleta Alexandru, Geanina Voicu, Cristina Ana Constantinescu, Daniela Rebleanu, Madalina Fenyo, Dan Simionescu, Agneta Simionescu, Ileana Manduteanu, Adriana Georgescu. Integrins α4β1 and αVβ3 are Reduced in Endothelial Progenitor Cells from Diabetic Dyslipidemic Mice and May Represent New Targets for Therapy in Aortic Valve Disease. Cell Transplantation. (2020). doi:10.1177/0963689720946277

  20. C Compton, J Canavan, J Mcleod, C Prevost, D Simionescu. “Reconstitution of the ventricular endocardium within acellular hearts”. Regen Eng Transl Med, (2019); 6(1): 90-100.

  21. HA Hussein, MM Harpa, I Movileanu, Hamida Al Hussein, H Suciu, K Branzaniuc, DT Simionescu. “Minimally Invasive Surgical Protocol for Adipose Derived Stem Cells Collection and Isolation - Ovine Model”. Rev. Chim., 70:5;1826-1828, (2019)

  22. MM Tucureanu, A Filippi, N Alexandru, CA Constantinescu, L Ciortan, R Macarie, M Vadana, G Voicu, S Frunza, D Nistor, A Simionescu, DT Simionescu, A Georgescu, I Manduteanu. “Diabetes-induced early molecular and functional changes in aortic heart valves in a murine model of atherosclerosis”. Diabetes and Vascular Disease Research (2019), 16;6:562-576.

  23. I Movileanu, K Brinzaniuc, MM Harpa, OS Cotoi, T Preda, HA Hussein, D Simionescu. “Cryopreservation increases availability and usability of ovine adipose-tissue derived stem cells for regenerative medicine research”. Revista de chimie (2019), in press.

  24. Filippi, N. Alexandru, G. Voicu, C.A. Constantinescu, M. Tucureanu, D. Rebleanu, M. Fenyo, D. Simionescu, A. Simionescu, I. Manduteanu, A. Georgescu. “Early Diabetes Induces Alterations In Endothelial Progenitor Cell Phenotype And Homing In Mice Susceptible To Atherosclerosis”, Atherosclerosis (2019); 287, e97.

  25. I Movileanu, K Brinzaniuc, A Chertes, T Preda, OS Cotoi, MM Harpa, HA Hussein, H Suciu, R Deac, M Portilla, D Simionescu. “A novel manufactured system designed to ensure consistent conditions in mechanical preconditioning of ovine adipose derived stem cells differentiation towards endothelial cell lineage”. Acta Medica Transilvanica (2019); 24, (3): 18-21.

  26. SS Patnaik, DT Simionescu, CJ Goergen, K Hoyt, S Sirsi, EA Finol. “Pentagalloyl glucose and its functional role in vascular health: biomechanics and drug-delivery characteristics”. Annals of BME, (2019); 47(1); 39-59. Full Text

  27. C Compton, J Canavan, J Mcleod, C Prevost, D Simionescu. “Reconstitution of the ventricular endocardium within acellular hearts”. Regen Eng Transl Med, (2019); epub ahead of print, April 8, 2019. Abstract

  28. HA Hussein, MM Harpa, I Movileanu, Hamida Al Hussein, H Suciu, K Branzaniuc, DT Simionescu. “Minimally Invasive Surgical Protocol for Adipose Derived Stem Cells Collection and Isolation - Ovine Model”. Rev. Chimie, 70:5;1826-1828, (2019). Full Text

  29. MM Tucureanu, A Filippi, N Alexandru, CA Constantinescu, L Ciortan, R Macarie, M Vadana, G Voicu, S Frunza, D Nistor, A Simionescu, DT Simionescu, A Georgescu, I Manduteanu. “Diabetes-induced early molecular and functional changes in aortic heart valves in a murine model of atherosclerosis”. Diabetes and Vascular Disease Research (2019); 16(6): 562-576. Full Text

  30. I Movileanu, K Brinzaniuc, MM Harpa, OS Cotoi, T Preda, HA Hussein, D Simionescu. “Cryopreservation increases availability and usability of ovine adipose-tissue derived stem cells for regenerative medicine research”. Revista de chimie (2019), (in press).

  31. A. Filippi, N. Alexandru, G. Voicu, C.A. Constantinescu, M. Tucureanu, D. Rebleanu, M. Fenyo, D. Simionescu, A. Simionescu, I. Manduteanu, A. Georgescu. “Early Diabetes Induces Alterations In Endothelial Progenitor Cell Phenotype And Homing In Mice Susceptible To Atherosclerosis”, Atherosclerosis (2019); 287, e97. Abstract

  32. I Movileanu, K Brinzaniuc, A Chertes, T Preda, OS Cotoi, MM Harpa, HA Hussein, H Suciu, R Deac, M Portilla, D Simionescu. “A novel manufactured system designed to ensure consistent conditions in mechanical preconditioning of ovine adipose derived stem cells differentiation towards endothelial cell lineage”. Acta Medica Transilvanica (2019); 24, (3): 18-21. Full Text

  33. G Lutter, H Dai, J Hansen, D Frank, A Haneya, D Simionescu, J Cremer, T Puehler. “Transcatheter mitral valve replacement (TMVR): annular or apical fixation?”. EuroIntervention, e-published on 03 Dec 2019. Full Text

  34. M. Thirugnanasambandam, D.T. Simionescu, P.G. Escobar, E. Sprague, B. Goins, G.D. Clarke, H.C Han, K.L. Amezcua, O.R. Adeyinka, C. J. Goergen, E. Finol. “The Effect of Pentagalloyl Glucose on the Wall Mechanics and Inflammatory Activity of Rat Abdominal Aortic Aneurysms”. J Biomech Eng. (2018); 140(8): 484-502. Full Text

  35. P. Olah, I. Movileanu, N. Suciu, M. Muji, M. Maruster, D. Simionescu, C. Avram. "Exploring Hierarchical Medical Data stored as Multi-trees in a Relational Database". (2017) IFMBE Proceedings, 59, 219-222. Abstract

  36. Haupt J, Lutter G, Gorb SN, Simionescu DT, Frank D, Seiler J, Paur A, Haben I. “Detergent-based decellularization strategy preserves macro- and microstructure of heart valves”. (2017) Int Cardiovasc Thorac Surg. 29155942

  37. Movileanu I, Harpa M, Brnazaniuc K, Suciu H, Cotoi O, Olah P, Simionescu D. “A standardized dissection protocol to generate aortic valvular scaffolds from porcine hearts”. (2017) Acta Medica Marisiensis., 63(3), 133-135. Abstract. Full Text PDF

  38. F Alexis, M Casco, T Olsen, A Herbst, G Evans, T Rothermel, L Pruett, D Simionescu,  R Visconti, "Iron Oxide Nanoparticles Stimulates Extra-Cellular Matrix Production into Cellular Spheroids." (2017) Bioengineering, 4(1). Direct Link

  39. Chow, J.P., Simionescu, D.T., Carter, A.L. A Simionescu. ”Immunomodulatory effects of adipose tissue-derived stem cells on elastin scaffold remodeling in diabetes". (2016) Tissue Eng Regen Med, 13, 701-712. Abstract. Full Text PDF

  40. M Harpa, I Movileanu, L Sierad, O Cotoi, H Suciu, T Preda, D Nistor, C Sircuta, K Branzaniuc, R Deac, S Gurzu, L Harceaga, P Olah, D Simionescu, M Dandel, A Simionescu. “In vivo testing of xenogeneic acellular aortic valves seeded with stem cells”. (2016) Romanian Review of Laboratory Medicine, 24(3), 343-346. Direct Link

  41. C Deborde, D Simionescu, J Liao, L Sierad, C Wright, A Simionescu. “Stabilized collagen and elastin-based scaffolds for mitral valve tissue engineering”. (2016) Tissue Engineering, Part A. 22(21-22), 1241-1251. 27608885, PMC5107718

  42. A Kennamer, L Sierad, R Pascal, N Rierson, C Albers, M Harpa, O Cotoi, L Harceaga, P Olah, P Terezia, A Simionescu, D Simionescu. “Bioreactor conditioning of valve scaffolds seeded internally with adult stem cells”. (2016) Tissue Engineering and Regenerative Medicine, 13(5), 507-515. Abstract

  43. M Harpa, I Movileanu, L Sierad, O Cotoi, H Suciu, C Sircuta, T Preda, D Nistor, K Branzaniuc, R Deac, M Dandel, S Gurzu, L Harceaga, P Olah, A Simionescu, D Simionescu. “Pulmonary heart valve replacement using stabilized acellular xenogeneic scaffolds; effects of seeding with autologous stem cells”. (Jan. 2016) In press, Romanian Review of Laboratory Medicine. Abstract

  44. M Harpa, I Movileanu, H Suciu, C Sircuta, K Branzaniuc, S Gurzu, L Harceaga, P Olah, A Simionescu, D Simionescu. “Mechanisms of failure of stem cell-seeded acellular valved conduits in a sheep right ventricular outflow tract model”. (Dec. 2015) Submitted to Acta Cardiologica Sinica.

  45. I Koleilat, M Jaeggli, J Ewing, M Androes, D Simionescu, J Eidt. “Interobserver variability in physician-modified endograft planning by comparison with a three-dimensional printed aortic model”. (2016) J Vasc Surg, 64(6), 1789-1796. 26607872

  46. L Sierad, EL Shaw, A Bina, B Brazile, N Rierson, S Patnaik, A Kennamer, R Odum, O Cotoi, T Preda, K Branzaniuc, H Smallwood, R Deac, I Egyed, Z Pavai, A Szanto, L Harceaga, H Suciu, V Raicea, P Olah, A Simionescu, J Liao, I Movileanu, M Harpa, D. Simionescu. “Functional heart valve scaffolds obtained by complete decellularization of porcine aortic roots in a novel differential pressure gradient perfusion system”. (2015) Tissue Engineering, Part C,21(12), 1284-1296. 26467108, PMC4663650

  47. M Harpa, K Branzaniuc, I Movileanu, O Cotoi, H Suciu, R Deac, L Harceaga, A Simionescu, D Simionescu. “Implantation of valvular collagen biomaterials seeded with autologous stem cells – intraoperative hemodynamic measurements in an animal model”. (2015). Acta Medica Transylvanica, 20(2), 38-39. Abstract

  48. L. Roudsari, C. Albers, T. Doucet, S. Floyd, C. Hoffman, K. Leaphart, E. Patrick, B. Roach, Ml Mahoney, L Sierad, J Nagatomi, D Simionescu. “Gelatin-Coated Resorbable Polymer Mesh as a Novel Scaffold for Aortic Heart Valve Tissue Engineering” (2014). Challenges in Regenerative Medicine, 1(1), 39-45. Direct Link

  49. D Simionescu. “Educational Ethics in Regenerative Medicine; a Hippocratic Oath for Biomedical Engineers”. (2014). Challenges in Regenerative Medicine, 1(1), 37-38. Direct Link

  50. LN Sierad, EL Shaw, R Launius, S McBride, C Storholt, R Poole, D Spence, K Miller, L Sosdian, K Allen, L Burton, A Iari, J Nagatomi, D Simionescu. “Toward an Endothelial-Cell Covered Mechanical Valve; Surface Re-Engineering and Bioreactor Testing of Mechanical Heart Valves”. (2014). Challenges in Regenerative Medicine, 1(1), 22-34 Direct Link

  51. T Pennel, G Fercana, D Bezuidenhout, A Simionescu, T Chuang, P Zilla, D Simionescu. “The Performance of Cross-linked Acellular Arterial Scaffolds as Vascular Grafts; Pre-clinical Testing in Direct and Isolation Loop Circulatory Models”. (2014).Biomaterials.  24816365, PMC4048826

  52. G. Fercana, D. Bowser, M. Portilla , E.M. Langan, C.G. Carsten, D.L. Cull, L.N. Sierad , D.T. Simionescu. “Platform technologies for decellularization, tunic-specific cell seeding and in vitro conditioning of extended length, small diameter vascular grafts”. (2014). E-pub, in press, Tissue Engineering, Part C. 24749889, PMC4241864

  53. Thomas Larrew, James Chow , Jason Schulte , Dan Simionescu, Agneta Simionescu. “The roles of various metabolic enzymes in diabetic cardiomyopathy—in vivo and in vitro approach”. Cardiovascular Pathology, (2013); 22(3):e48. Abstract

  54. Mattix, B., Olsen, T., Casco, M., Herbst, A., Simionescu, D., Visconti, R., Alexis, F. “Biological magnetic cellular spheroids as building blocks for tissue engineering.” Acta Biomaterialia. (2014); 10(2): 623-629. 24176725, PMC4733563

  55. Mattix, B., Moore, T., Olsen, T., Simionescu, D., Visconti, R., Alexis, F. “Accelerated iron oxide nanoparticle degradation via polymer encapsulation for tissue engineering.” Advanced Functional Materials. (2014); 24(6): 800-807.  Abstract

  56. Mattix, B., Poole, J., Casco, M., Uvarov, O., Simionescu, D., Alexis, F. ”Small Diameter Blood Vessels: Replacement Strategies, Maturation Techniques, and Vascular Network Formation”. Journal of Tissue Science & Engineering, (2013), 4(2),1. Abstract

  57. George Fercana, Eugene Langan, Christopher Wright, Dan Simionescu. “Development of living vascular grafts for peripheral bypass applications using a novel trans-mural perfusion bioreactor”. Cardiovascular Pathology, (2013), 22(3):e35. Abstract

  58. JJ Mercuri, C Addington, R Pascal, S Gill, D Simionescu. "Development and initial characterization of a chemically stabilized elastin-glycosaminoglycan-collagen composite shape-memory hydrogel for nucleus pulposus regeneration." Journal of Biomedical Materials Research, Part A. Epub ahead of print. (2014). 24497431

  59. JP Chow, DT Simionescu, H Warner , B Wang, SS Patnaik, J Liao, A Simionescu. "Mitigation of Diabetes-Related Complications in Implanted Collagen and Elastin Scaffolds Using Matrix-Binding Polyphenol". Biomaterials, 34(3): 685-695 (2013) 23103157, PMC3496025
  60. JJ Mercuri, S Patnaik, SS Gill, J Liao, DT Simionescu. "Regenerative Potential of Decellularized Porcine Nucleus Pulposus Hydrogel Scaffolds; Stem cell Differentiation, Matrix Remodeling and Biocompatibility Studies". Tissue Engineering, Part A., 19(7-8): 952-66 (2013) 23140227

  61. JB Schulte, A Simionescu, DT Simionescu. "The Acellular Myocardial Flap; a Novel Extracellular Matrix Scaffold Enriched with Patent Microvascular Networks and Biocompatible Cell Niches". Tissue Engineering, Part C.,19(7): 518-30 (2013). 23151037, PMC3662386

  62. B Wang, ME Tedder, CE Perez, G Wang, AL de Jongh Curry, F To, SH Elder, LN Williams, DT Simionescu, J Liao. "Structural and Biomechanical Characterizations of Porcine Myocardial Extracellular Matrix". Journal of Materials Science: Materials in Medicine, 23(8):1835-1847 (2012). 22584822, PMC3523096

  63. Dan T. Simionescu, Joseph Chen, Michael Jaeggli, Jun Liao. “Form Follows Function: Advances in Trilayered Structure Replication for Aortic Heart Valve Tissue Engineering.” Journal of Healthcare Engineering, 3(2): 179-202 (2012) 23355946, PMC3552623

  64. Agneta Simionescu,  Jason Schulte, George Fercana and D. Simionescu. "Inflammation in Cardiovascular Tissue Engineering: the Challenge to a Promise". International Journal of Inflammation. (2011). 21755031, PMC3132660

  65. Agneta Simionescu, Mary E. Tedder, Ting-Hsien Chuang, D. Simionescu. “Lectin and Antibody-Based Histochemical Techniques For Cardiovascular Tissue Engineering”. Journal of Histotechnology, 34(1): 20-28 (2011). Abstract

  66. Tedder ME, Liao J, Simionescu A, Simionescu DT, “Assembly and testing of layered collagen constructs for heart valve tissue engineering”. Tissue Engineering, Part A, 17(1-2): 25-36. (2011). 20673028, PMC3011922

  67. Jeremy J. Mercuri,Sanjitpal S. Gill, Dan T. Simionescu, “Novel Tissue Derived Biomimetic Scaffold for Regenerating the Human Nucleus Pulposus”. Journal of Biomedical Materials Research, part A. Feb, 96A(2): 422–435. (2011). 21171162

  68. M.B. Ariganello, D. Simionescu, R.S. Labow, J. Michael Lee, “Macrophage Differentiation and Polarization on a Decellularized Pericardial Biomaterial”. Biomaterials, 32(2): 439-49 (2011). 20933269, PMC3568399

  69. Jeremy J. Mercuri , Sanjitpal S. Gill, D. Simionescu, “Novel Tissue-Derived Biomimetic Scaffolds for Regenerating the Human Nucleus Pulposus”, e-pub, Journal of Biomaterial Materials Research, part A., 96(2): 422-35 (2011). 21154457

  70. Leslie N. Sierad, Christopher Albers, Jordan Maivelett, Mary E. Tedder, Jun Liao, D. Simionescu, Agneta Simionescu, “Endothelialization of decellularized penta-galloyl-glucose treated xenograft scaffolds and dynamic conditioning in a heart valve bioreactor”, Cardiovascular Engineering and Technology, 1(2): 138-153 (2010).

  71. Bo Wang, Ali Borazjani, Mina Tahai, Amy L. de Jongh Curry, D. Simionescu, Jianjun Guan, Filip To, Steve H. Elder, Jun Liao, “Fabrication of Cardiac Patch with Decellularized Porcine Myocardial Scaffold and Bone Marrow Mononuclear Cells”, Journal of Biomaterial Materials Research, part A., 94(4): 1100-1110 (2010). 20694977, PMC2933781

  72. M. E. Plonska-Brzezinska, A. Palkar, K. Winkler, A. Simionescu, D. Simionescu, A. Dubis, A. J. Athans, L. Echegoyen, “Small non-cytotoxic carbon nano-onions: investigation of the electrochemical behavior and their potential application”, Chemistry, 16(16): 4870-4880. (2010).

  73. Jeremy J. Mercuri, Sanjitpal S. Gill, D. Simionescu, “Novel Tissue-Derived Biomimetic Scaffolds for Regenerating the Human Nucleus Pulposus”, Journal of Biomaterial Materials Research, part A., 96(2): 422-35 (2011). 21171162

  74. T. Chuang, C. Stabler, A. Simionescu, D. Simionescu, “Polyphenol-stabilized tubular elastin scaffolds for tissue engineered vascular grafts”, Tissue Engineering Part A, 15(10): 2837-2851 (2009). 19254115, PMC2792047

  75. M. E. Tedder, J. Liao, B. Weed, C. Stabler, H. Zhang, A. Simionescu, D. Simionescu, “Stabilized collagen scaffolds for heart valve tissue engineering”, Tissue Engineering Part A, 15(6): 1257-1268 (2009). 18928400, PMC2792094

  76. Y.Y. Yuan, P.J. Kostenuik, S. Morony, D. Simionescu, D.M. Basalyga, T.A. Bateman, “Skeletal deterioration induced by RANKL infusion; a model for high-turnover bone disease”, Osteoporosis International, 19(5): 625-35 (2008). 18038244

  77. Simionescu A, Simionescu D, Vyavahare N, “Osteogenic responses in fibroblasts activated by elastin degradation products and transforming growth factor-beta1: role of myofibroblasts in vascular calcification”, American Journal of Pathology, 171(1): 116-23 (2007). 17591959, PMC1941602

  78. Raghavan D, Simionescu D, Vyavahare N, “Neomycin prevents enzyme-mediated glycosamino-glycan degradation in bioprosthetic heart valves”, Biomaterials, 28(18): 2861-8 (2007). 17353047, PMC2262162

  79. Kurane A, Simionescu D, Vyavahare N, “In vivo cellular repopulation of tubular elastin scaffolds mediated by basic fibroblast growth factor”, Biomaterials, 28(18): 2830-2838 (2007). 17368531, PMC2262161

  80. Isenburg J, Simionescu D, Starcher B, Vyavahare N, “Elastin stabilization for treatment of abdominal aortic aneurysms”, Circulation, 115(13): 1729-37 (2007). 17372168. Full Text

  81. Mercuri J, Lovekamp J, Simionescu D, Vyavahare NR, “Glycosaminoglycan-targeted fixation for improved bioprosthetic heart valve stabilization”, Biomaterials, 28(3): 496-503 (2007). 17030363

  82. Simpson CL, Lindley S, Eisenberg C, Basalyga DM, Starcher BC, Simionescu D, Vyavahare NR, “Toward cell therapy for vascular calcification: osteoclast-mediated demineralization of calcified elastin”, Cardiovascular Pathology, 16(1): 29-37 (2007). 17218212

  83. Kasyanov V, Isenburg JC, Draughn AR, Hazard S, Hodde J, Iveta O, Halkes B, Pieters R, Simionescu D, Markwald RR, Mironov V, “Tannic acid mimicking dendrimers as a small intestine submucosa stabilizing nano-agent”, Biomaterials, 27(5): 745-751 (2006). 16102811

  84. Isenburg JC, Simionescu D, Ramachandani N, Vyavahare NR, “Structural requirements for stabilization of vascular elastin by polyphenolic tannins”, Biomaterials, 27(19): 3645-3651 (2006). 16527345

  85. Lee JS, Basalyga D, Simionescu D, Vyavahare NR, “Elastin calcification in the rat subdermal model is accompanied by up-regulation of degradative and osteogenic cellular responses”, American Journal of Pathology, 168(2): 490-498 (2006). 16436663, PMC1606489

  86. Isenburg J, Simionescu D, Vyavahare N, "Tannic acid treatment enhances biostability and reduces calcification of glutaraldehyde fixed aortic wall", Biomaterials, 26(11): 1237-1245 (2005). 15475053

  87. Lovekamp JJ, Simionescu D, Mercuri J, Zubiate B, Sacks M, Vyavahare NR, “Stability and function of glycosaminoglycans in porcine bioprosthetic heart valves”, Biomaterials, 27 (8): 1507-1518 (2005). 16144707, PMC2262164

  88. Simionescu D, Lu Q, Song Y, Lee JS, Rosenbalm TN, Kelley C, Vyavahare NR, "Biocompatibility and remodeling potential of pure arterial elastin and collagen scaffolds", Biomaterials, 27 (5), 702-713 (2005). 16048731

  89. Isenburg JC, Simionescu D, Vyavahare NR, "Elastin stabilization in cardiovascular implants: improved resistance to enzymatic degradation by treatment with tannic acid", Biomaterials, 25(16): 3293-3302 (2004). 14980424

  90. Bailey M, Pillarisetti S, Jones P, Xiao H, Simionescu D, Vyavahare N, "Involvement of matrix metalloproteinases and tenascin-C in elastin calcification", Cardiovascular Pathology, 13(3): 146-155 (2004). 15081471

  91. Lu Q, Ganesan K, Simionescu D, Vyavahare NR, "Novel porous aortic elastin and collagen scaffolds for tissue engineering", Biomaterials, 25(22): 5227-5237 (2004). 15110474

  92. Basalyga DM, Simionescu D, Xiong W, Baxter BT, Starcher BC, Vyavahare NR, "Elastin degradation and calcification in an abdominal aorta injury model: role of matrix metalloproteinases", Circulation, 110(22): 3480-3487 (2004). 15545515,
    PMC1262646

  93. Simionescu D, "Prevention of calcification in bioprosthetic heart valves: challenges and perspectives". By invitation. Expert Opinions in Biological Therapy, 4(12), 1971-1985 (2004). 15571459

  94. Simionescu D, Lovekamp JJ, Vyavahare NR, "Extracellular matrix degrading enzymes are active in porcine stentless aortic bioprosthetic heart valves". Journal of Biomedical Materials Research, 66(4): 755-763 (2003). 12926026

  95. D. Simionescu, J. Lovekamp, N. Vyavahare, “Glycosaminoglycan-degrading enzymes in porcine aortic heart valves; implications for bioprosthetic heart valve degeneration". Journal of Heart Valve Disease, 12(2): 217-225 (2003). 12701795

  96. D. Simionescu, J. Lovekamp, N. Vyavahare, “Degeneration of stentless bioprosthetic heart valves is initiated during tissue preparation; an ultrastructural study. Journal of Heart Valve Disease, 12(2): 226-234 (2003). 12701796

  97. M. Bracher, D. Simionescu, A. Simionescu, N. Davies, P. Human, P. Zilla, “The role of matrix metalloproteinases in tissue valve degeneration – a mini-review”. Journal of Long Term Effects of Medical Implants, 11(3-4): 221-230 (2001).

  98. A. Simionescu, D. Simionescu, R. Deac, "Biochemical pathways of tissue degeneration in bioprosthetic cardiac valves; the role of matrix metalloproteinases". ASAIO Journal, 42(5): M561-M567 (1996). 8944942

  99. A. Simionescu, D. Simionescu, R. Deac, "Matrix metalloproteinases in the pathology of natural and bioprosthetic cardiac valves". Cardiovascular Pathology, 5(6): 323-332 (1996). Abstract

  100. R. Deac, D. Simionescu, D. Deac, "New evolution in mitral physiology and surgery; mitral stentless pericardial valve", Annals of Thoracic Surgery, 60(2 Supp): S433-S438 (1995). 7646203

  101. D. Simionescu, R. Deac, A. Simionescu, "Inhibition of extracellular-matrix degrading proteases in pericardial derived cardiovascular bioprostheses", Journal of Material Science; Materials in Medicine, 5(9-10): 748-751 (1994). Abstract

  102. D. Simionescu, A. Simionescu, R. Deac, "Remnant proteolytic ac­tivities in glutaraldehyde treated bovine pericardium and in ex­planted cardiac bioprostheses", Journal of Biomedical Materials Research, 27(6): 821-829 (1993). 8408112

  103. D. Simionescu, A. Simionescu, R. Deac. "Mapping of glutaraldehyde-treated bovine pericardium for use as bioprosthetic cardiac valves", Journal of Biomedical Materials Research,27 (6): 697-704 (1993). 8408100

  104. Simionescu A, D. Simionescu, R. Deac. "Lysine-enhanced glutaral­dehyde crosslinking of collagenous biomaterials", Journal of Biomedical Materials Research, 25(12): 1495-1505 (1991). 1794997

  105. D. Simionescu, N.A.  Kefalides. "Biosynthesis of proteoglycans and interstitial collagens by bovine pericardial fibroblasts", Experimental Cell Research, 195(1), 171-176 (1991). 2055265

  106. D. Simionescu, R.V. Iozzo and N.A. Kefalides. "Bovine pericardial proteoglycan; biochemical, immunochemical and ultrastructural studies", Matrix, 9(4): 301-310 (1989). 2677626

  107. D. Simionescu, R. Alper and N.A. Kefalides. "Partial characterization of a low molecular weight proteogly­can isolated from bovine parietal pericardium". Biochemical and Biophysical Research Communications, 151(1): 480-486 (1988). 3348789

  108. D. Simionescu, M. Simionescu. "Differentiated distribution of the cell surface charge on the alveolar capillary unit".  Microvascular Research, 25(1): 85-100 (1983). 6835101
Department of Bioengineering | 301 Rhodes Research Center, Clemson, SC 29634-0905