TISSUE ENGINEERING LAB


Journal Publications

  1. Dan Simionescu, Nishanth Tharayil, Elizabeth Leonard, Wenda Carlyle, Alex Schwarz, Kelvin Ning, Christopher Carsten, Juan Carlos Carrillo Garcia*, Alexander Carter*, Collin Owens*, Agneta Simionescu. “Binding of pentagalloyl glucose to aortic wall proteins: insights from peptide mapping and simulated docking studies”. Bioengineering, 2023,10 (8), 936.
  2. Bo Wang, Leslie N Sierad, Jeremy J Mercuri, Agneta Simionescu, Dan T Simionescu, Lakiesha N Williams, Ryan Vela, Pietro Bajona, Matthias Peltz, Sharan Ramaswamy, Yi Hong, Jun Liao. “Structural and biomechanical characterizations of acellular porcine mitral valve scaffolds: anterior leaflets, posterior leaflets, and chordae tendineae.” Engineered regeneration, 2022, 3 (4), 374-386.
  3. A Filippi, A Constantin, N Alexandru, CA Mocanu, ML Vlad, IM Fenyo, Agneta Simionescu, Dan Teodor Simionescu, Ileana Manduteanu, Adriana Georgescu, “VLA4-Enhanced Allogeneic Endothelial Progenitor Cell-Based Therapy Preserves the Aortic Valve Function in a Mouse Model of Dyslipidemia and Diabetes”. Pharmaceutics, 2022, 14 (5), 1077.
  4. Movileanu I, Nistor D, Sierad L, Brinzaniuc K, Marius Mihai Harpa, Terezia Preda, Ovidiu Cotoi, Hussam Al Hussein, Hamida Al Hussein, Alexandru Chertes, Horatiu Suciu, Agneta Simionescu, Dan Simionescu, “Developing the Tissue Engineered Heart Valve – a Descriptive Hemodynamic and Ultrasound in Vitro Characterization Study of Heart Valves in a Bioreactor. Romanian Journal of Cardiology, 2021, Vol 31 (3).
  5. Simionescu D, Harpa M, Agneta Simionescu, Oprita A, Movileanu I, “Tissue Engineering Heart Valves - a Review of More than Two Decades into Preclinical and Clinical Testing for Obtaining the Next Generation of Heart Valve Substitutes” Romanian Journal of Cardiology, 2021, 31 (3).
  6. Spencer Marsh*, Madeline Raudat*, Bethany Lefeber*, Laura Beth Herndon*, Howard Herbert*, Laura McCallum*, Agneta Simionescu. “Dynamic bioreactor model to mimic early cardiac fibrosis in diabetes.” Journal of Mechanics in Medicine and Biology, 2021, 21(6):2150047.
  7. Ionela Movileanu, Marius Harpa, Hussam Al Hussein, Lucian Harceaga, Alexandru Chertes, Hamida Al Hussein, Georg Lutter, Thomas Puehler, Terezia Preda, Carmen Sircuta, Ovidiu Cotoi, Dan Nistor, Adrian Man, Bogdan Cordos, Radu Deac, Horatiu Suciu, Klara Brinzaniuc, Megan Casco*, Leslie Sierad*, Margarita Bruce*, Dan Simionescu, Agneta Simionescu. “Pre-clinical Testing of Living Tissue Engineered Heart Valves for Pediatric Patients, Challenges and Opportunities”. Frontiers in Cardiovascular Medicine, section Heart Valve Disease. 2021, Vol. 8, article 707892.
  8. Georg Lutter, Lennart Bax, Yazhou Liu, Jan-Hinnerk Hansen, Derk Frank, Sandra Freitag-Wolf, Agneta Simionescu, Janarthanan Sathananthan, Thomas Puehler. Transcatheter mitral valve replacement: tissue in-growth after 4 weeks. Interactive CardioVascular and Thoracic Surgery, 2021, 32(1):1–8.
  9. Ileana Manduteanu, Dan Simionescu, Agneta Simionescu, Maya Simionescu. “Aortic Valve Disease in Diabetes: Molecular Mechanisms and Novel Therapies”. Journal of Cellular and Molecular Medicine. 2021; 25(20):9483-9495.
  10. Cecoltan S, Ciortan L, Macarie RD, Vadana M, Mihaila AC, Tucureanu M, Vlad M-L, Droc I, Gherghiceanu M, Simionescu Agneta, Simionescu DT, Butoi E and Manduteanu I. High Glucose Induced Changes in Human VEC Phenotype in a 3D Hydrogel Derived from Cell-Free Native Aortic Root. Front. Cardiovasc. Med.. 2021, Vol. 8, article 714573.
  11. Filippi A, Constantin A, Alina Constantin, Nicoleta Alexandru, Geanina Voicu, Cristina Ana Constantinescu, Daniela Rebleanu, Madalina Fenyo, Dan Simionescu, Agneta Simionescu, Ileana Manduteanu, Adriana Georgescu. “Integrins α4β1 and αVβ3 are Reduced in Endothelial Progenitor Cells from Diabetic Dyslipidemic Mice and May Represent New Targets for Therapy in Aortic Valve Disease.” Cell Transplantation. 2020. doi:10.1177/0963689720946277.
  12. MM Tucureanu, A Filippi, N Alexandru, CA Constantinescu, L Ciortan, R Macarie, M Vadana, G Voicu, S Frunza, D Nistor, Agneta Simionescu, DT Simionescu, A Georgescu, I Manduteanu. “Diabetes-induced early molecular and functional changes in aortic heart valves in a murine model of atherosclerosis”. Diabetes and Vascular Disease Research 2019. 16(6):562-576.
  13. J Dhulekar, A Simionescu. “Challenges in vascular tissue engineering for diabetic patients”. Acta Biomaterialia. (2018); 70:25-34.
  14. J Chow, D Simionescu, AL Carter, A Simionescu. “Immunomodulatory effects of autologous adipose tissue-derived stem cells on vascular scaffold remodeling in diabetes”. Tissue Engineering and Regenerative Medicine. (2016); 13(6): 701-712.
  15. C Deborde, D Simionescu, J Liao, L Sierad, C Wright, A Simionescu. “Stabilized collagen and elastin-based scaffolds for mitral valve tissue engineering”. Tissue Engineering, Part A. (2016); 22(21-22):1241-1251.
  16. M Harpa, I Movileanu, L Sierad, O Cotoi, H Suciu, T Preda, D Nistor, C Sircuta, K Branzaniuc, R Deac, S Gurzu, L Harceaga, P Olah, D Simionescu, M Dandel, A Simionescu. “In vivo testing of xenogeneic acellular aortic valves seeded with stem cells”. Romanian Review of Laboratory Medicine. (2016); 24(3). In press.
  17. Chawla V, Simionescu A, Langan EM III, LaBerge M. “Influence of clinically relevant mechanical forces on vascular smooth muscle cells under chronic high glucose: An In Vitro Dynamic Disease Model”. Annals of Vascular Surgery. (2016); 34: 212-226.
  18. A Kennamer, L Sierad, R Pascal, N Rierson, C Albers, M Harpa, O Cotoi, L Harceaga, P Olah, P Terezia, A Simionescu, D Simionescu. “Bioreactor conditioning of valve scaffolds seeded internally with adult stem cells”. Tissue Engineering and Regenerative Medicine. (2016); 13(5): 507-515.
  19. M Harpa, I Movileanu, L Sierad, O Cotoi, H Suciu, C Sircuta, T Preda, D Nistor, K Branzaniuc, R Deac, M Dandel, S Gurzu, L Harceaga, P Olah, A Simionescu, D Simionescu. “Pulmonary heart valve replacement using stabilized acellular xenogeneic scaffolds; effects of seeding with autologous stem cells”. Romanian Review of Laboratory Medicine. (2015); 23(4).
  20. L Sierad, EL Shaw, A Bina, B Brazile, N Rierson, S Patnaik, A Kennamer, R Odum, O Cotoi, T Preda, K Branzaniuc, H Smallwood, R Deac, I Egyed, Z Pavai, A Szanto, L Harceaga, H Suciu, V Raicea, P Olah, A Simionescu, J Liao, I Movileanu, M Harpa, D. Simionescu. “Functional heart valve scaffolds obtained by complete decellularization of porcine aortic roots in a novel differential pressure gradient perfusion system”. Tissue Engineering, Part C. (2015); 21(12); 1284-1296.
  21. M Harpa, K Branzaniuc, I Movileanu, O Cotoi, H Suciu, R Deac, L Harceaga, A Simionescu, D Simionescu. “Implantation of valvular collagen biomaterials seeded with autologous stem cells – intraoperative hemodynamic measurements in an animal model”. Acta Medica Transylvanica. (2015); 20(2): 38-39.
  22. T Pennel, G Fercana, D Bezuidenhout, A Simionescu, T Chuang, P Zilla, D Simionescu. “The Performance of Cross-linked Acellular Arterial Scaffolds as Vascular Grafts; Pre-clinical Testing in Direct and Isolation Loop Circulatory Models”. Biomaterials. (2014); 35(24); 6311-22.
  23. Mattix BM, Olsen TR, Casco M, Reese L, Poole JT, Zhang J, Visconti RP, Simionescu A, Simionescu DT, Alexis F. "Janus magnetic cellular spheroids for vascular tissue engineering". Biomaterials. (2014); 35(3): 949-960.
  24. T Larrew, J Chow, J Schulte, D Simionescu, A Simionescu. “The roles of various metabolic enzymes in diabetic cardiomyopathy—in vivo and in vitro approach”. Cardiovascular Pathology. (2013); 22(3): e48.
  25. JB Schulte, A Simionescu, DT Simionescu. “The Acellular Myocardial Flap; a Novel Extracellular Matrix Scaffold Enriched with Patent Microvascular Networks and Biocompatible Cell Niches”. Tissue Engineering. (2013); 19(7): 518-530.
  26. JP Chow, DT Simionescu, H Warner, B Wang, SS Patnaik, J Liao, A Simionescu. “Mitigation of Diabetes-Related Complications in Implanted Collagen and Elastin Scaffolds Using Matrix-Binding Polyphenol”. Biomaterials. (2013); 34(3): 685-695.
  27. D Simionescu, M Jaeggli, J Liao, A Simionescu. “Translational personalized regenerative medicine; scaffolds and stem cells for patient-tailored aortic heart valve tissue engineering”. Cardiology (2012); 121: 138.
  28. Tedder ME, Liao J, Simionescu A, Simionescu DT, “Assembly and testing of layered collagen constructs for heart valve tissue engineering”. Tissue Engineering, Part A. (2011); 17(1-2): 25-36. 20673028.
  29. A Simionescu, Mary E. Tedder, Ting-Hsien Chuang, D. Simionescu. “Lectin and Antibody-Based Histochemical Techniques For Cardiovascular Tissue Engineering”. Journal of Histotechnology (April 2011); 34(1): 20-28.
  30. A. Simionescu, JB Schulte, G Fercana, DT Simionescu, “Inflammation in Cardiovascular Tissue Engineering: The Challenge to a Promise: A Minireview”. International Journal of Inflammation, vol. 2011 (2011); Article ID 958247, 11 pages.
  31. Leslie N. Sierad, Christopher Albers, Jordan Maivelett, Mary E. Tedder, Jun Liao, D. Simionescu, A Simionescu, “Endothelialization of decellularized penta-galloyl-glucose treated xenograft scaffolds and dynamic conditioning in a heart valve bioreactor”. Cardiovascular Engineering and Technology (2010); 1(2): 138-153.
  32. M. E. Plonska-Brzezinska, A. Palkar, K. Winkler, A. Simionescu, D. Simionescu, A. Dubis, A. J. Athans, L. Echegoyen, “Small non-cytotoxic carbon nano-onions: investigation of the electrochemical behavior and their potential application”. Chemistry (2010); 16(16): 4870-4880.
  33. Tedder ME, Liao J, Weed B, Stabler C, Zhang H, Simionescu A, Simionescu DT. “Stabilized collagen scaffolds for heart valve tissue engineering”. Tissue Engineering Part A. (2009); 15(6): 1257-1268.
  34. Chuang TH, Stabler C, Simionescu A, Simionescu DT. “Polyphenol-stabilized tubular elastin scaffolds for tissue engineered vascular grafts”. Tiss Eng Part A. (2009); 15(10): 1257-1268.
  35. Simionescu A, Simionescu D, Vyavahare NR. “Osteogenic responses in fibroblasts activated by elastin degradation products and TGF-ß1: role of myofibroblasts in vascular calcification”. American Journal of Pathology (2007); 171(1): 116-23.
  36. Lee JS, Basalyga DM, Simionescu A, Isenburg JC, Simionescu D, Vyavahare NR. “Elastin Calcification in the Rat Subdermal Model Is Accompanied by Up-Regulation of Degradative and Osteogenic Cellular Responses”. American Journal of Pathology (2006); 168(2): 490-8.
  37. Lu Q, Simionescu A, Vyavahare NR. “Novel capillary channel fiber scaffolds for guided tissue engineering”. Acta Biomater. (2005 Nov); 1(6): 607-14.
  38. Simionescu A, Philips K, Vyavahare N. "Elastin-derived peptides and TGF-beta1 induce osteogenic responses in smooth muscle cells". Biochem Biophys Res Commun. (2005); 334(2): 524-532.
  39. Simionescu A, Simionescu D, Deac R. "Matrix metalloproteinases in the pathology of natural and bioprosthetic cardiac valves". Cardiovascular Pathology (1996); 5: 323-332.
  40. Simionescu D, Deac R, Simionescu A. "Inhibition of extracellular-matrix degrading proteases in pericardial derived cardiovascular bioprostheses". Journal of Material Science; Materials in Medicine (1994); 5 (9-10): 748-751.
  41. Simionescu A, Simionescu D, Deac R. "Lysine-enhanced glutaraldehyde crosslinking of collagenous biomaterials". Journal of Biomedical Materials Research (1991); 25(12): 1495-1505.

Books and Monographs

  1. Dan Simionescu, Agneta Simionescu, book editors. InTech - Open Access Publisher, Rijeka, Croatia. Title, “Physiologic and Pathologic Angiogenesis - Signaling Mechanisms and Targeted Therapy”. ISBN 978-953-51-3023-6. (April 2017).
  2. Dan Simionescu, Agneta Simionescu, book editors. InTech - Open Access Publisher, Rijeka, Croatia. Title, “Vasculogenesis and Angiogenesis – from Embryonic Development to regenerative Medicine”. ISBN 978-953-307-882-3. (November 2011).
  3. Simionescu D, Simionescu Agneta, Deac R, "Molecular pathology of biomaterials, in "Cell adhesion and the extracellular matrix". C. Cruce, Agneta Simionescu, D. Simionescu et.al (eds), AIUS Publishers, Craiova, Romania, 1997 (in Romanian).

Patents

  1. Modular bioreactor, compliance chamber for a bioreactor, and cell seeding apparatus. L Sierad, C Delaney, R Pascal III, D Simionescu, A Simionescu. 2023. US Patent 11,034,928US
  2. Continuation Patent Application Entitled: “Modular Bioreactor, Compliance Chamber for a Bioreactor, and Cell Seeding Apparatus”, L Sierad, D Simionescu, A Simionescu. Application No.: 17/244,447; Filed: April 29, 2021. Our Ref: CXU-809-CON; Your Ref: 2014-056
  3. US Patent Issued US10660754B2 "Self-adjusting tissue holder", L Sierad, R Pascal III, C Deborde, D Simionescu, A Simionescu, 2020
  4. “Self-adjusting tissue holder”. US Patent #10,022,225, 2018, with L. Sierad, R. Pascal, C deBorde, D Simionescu
  5. "Treatment to Render Implants Resistant to Diabetes”. US Patent # 9,283,241, 2016, A Simionescu, with James Chow and D. Simionescu.
  6. “Method and kit for rapid preparation of autologous tissue medical devices”. US/World, Patent #6,214,055, 2001, A Simionescu with D. Simionescu and R. Deac