Opportunistic bacterial pathogens (OBPs) commonly colonize the internal surfaces of premising plumbing pipes and fixtures, and can cause respiratory infections in immune-compromised individuals. In particular, inhalation of water aerosols during showering is a known exposure route for Legionella and Mycobacteria infections. Incorporation of UVC LEDs into showerheads is a newly proposed preventative technology; however little is known about the capability of UVC irradiation to inactivate OBPs within complex biological matrices or to prevent biofilm establishment. We have developed methods for monitoring and quantifying biofilm inhibition by continuous UVC exposure of surfaces and are conducting experiments that will aid in the design of future UV-equipped showerheads.